DETECTION OF AUTO- AND ALLOANTIBODIES AGAINST GRANULOCYTES BY MEANS OF GEL AGGLUTINATION TECHNIQUE

Abstract

Аbstract. Granulocyte antigen-specific autoantibodies may be implicated into pathogenesis of autoand alloimmune neutropenia, neonatal alloimmune neutropenia, and acute pulmonary insufficiency occurring post-transfusion. However, detection and identification of clinically significant granulocyte antibodies still represent a technically difficult task. To detect immunoglobulins and/or activated complement on granulocyte surface, we have adapted a Gel Test (ID Micro Typing System, DiaMed, Switzerland) which is commonly applied for diagnostics of RBC-specific antibodies. Our results suggest that the data from this gel test are quite reproducible, and the system may be used for detection of granulocyte-specific antibodies belonging to different immunoglobulin classes. (Med. Immunol., 2011, vol. 13, N 2-3, pp 253-256)

Keywords

• neutropenia
• granulocytes
• antigens
• antibodies
• immunoglobulin