Derivation of LIF-independent mouse iPS cells with modified Oct4

Hiroyuki Hirai; Meri Firpo; Nobuaki Kikyo

doi:10.1016/j.scr.2015.08.005

Stem Cell Research (Sep 2015)

Derivation of LIF-independent mouse iPS cells with modified Oct4

Hiroyuki Hirai,
Meri Firpo,
Nobuaki Kikyo

Affiliations

Hiroyuki Hirai: Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA
Meri Firpo: Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA
Nobuaki Kikyo: Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA

DOI: https://doi.org/10.1016/j.scr.2015.08.005
Journal volume & issue: Vol. 15, no. 2
pp. 384 – 386

Abstract

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It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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