Armaghane Danesh Bimonthly Journal (Jul 2023)
Evaluation of anti-proliferative and anti-cancer properties of hydroalcoholic extract of Jaft and Cisplatin on AGS cell line of gastric cancer
Abstract
Abstract Background: Oak placenta(jaft) extract has potent antioxidant, anti-proliferative and anti-cancer activity, and other therapeutic properties. The aim of this study is to investigate the effect of the hydroalcoholic extract of the Jaft on the cytotoxicity of cisplatin on the AGS gastric adenocarcinoma cell line. Materials and methods: The MTT method was used to check cell viability and cell viability after treatment with jaft extract and cisplatin. Compusyn software was used to check the combined index of these two materials. The real-time PCR method was used to express the CDK2 gene as an important gene involved in the cell cycle. Statistical analysis was done using Graph Pad Prism 6 software and a one-way ANOVA statistical method Results : The findings of the present study showed that Jaft extract and cisplatin cause decreased cell viability of AGS, and the analysis of compusyn data showed that these two substances together have synergistic properties (CI<1), as well as simultaneous use of Jaft extract increases the cytotoxicity of cisplatin drug. In the simultaneous treatment, it was observed that the expression of CDK2 decreased and the anticancer effect of cisplatin increased(p<***). Therefore, the synergistic effects of these two drugs can be dependent on CDK2 expression. Conclusion: The present study shows that jaft extract has anti-proliferative and anti-cancer properties. suggests a possible approach to treat gastric cancer with higher potency and lower dose of cisplatin to induce reduced cell viability. a proper selection to sensitize cancer cells to cisplatin.
