Fecal aminoacid profile in infants depending on their age and sex characteristics, type of feedingand allergic anamnesis

Abstract

Objectives. To study the content of fecal amino acids in infants depending on their age and sex characteristics, as well as type of feeding and family allergic anamnesis. Material and methods. 31 children aged from 1 month to 1 year were selected for participation in the study. The mothers of these children were instructed in detail about the procedure of collecting stool samples from their infants. Qualitative and quantitative determination of free amino acids and their derivatives was carried out by high performance liquid chromatography using the HP-Agilent 1100 liquid chromatograph (Agilent Technologies, USA). Results. Concentrations of β-aminobutyric acid, 3-methylhistidine, β-alanine and ornithine in children of the second half of their life were higher than in those in the first 6 months of life. In children of the first half of life, the concentrations of β-aminobutyric acid and 3-methylhistidine were below the threshold level of determination, after 6 months the content of these amino acids increased. Boys had a higher concentration of ethanolamine and ornithine in the stool than girls. Children on artificial feeding had significantly lower taurine numbers. In children at risk for allergic diseases, the concentration of tryptophan was lower than in children with an unburdened history of allergies. Conclusions. With age, the concentration of 3-methylhistidine, β-alanine, β-aminobutyric acid and ornithine increases in the stool. In boys, the concentration of ethanolamine and ornithine is higher than in girls. Artificially fed infants have lower fecal taurine concentrations than breastfed ones. The level of tryptophan in children at risk for allergies was lower than in the group of children with an unburdened allergic anamnesis.

Keywords

• microbiota
• children
• fecal markers
• amino acids
• allergy
• nutrition