Tissue culture-based preparation and validation of the turkey pox virus vaccine

Abstract

Turkey Pox Virus (TKPV) is one of avipox virus affecting poultry breeding causing many economic losses due to general skin lesion on the non-feathered area of the body (cutaneous form) and may be fatal in case of diphtheritic form. Turkey breeding in Egypt is getting a lot of attention lately so the disease of turkey get more concern, the most important disease that causes loses is (TKPV). The most effective way to control the disease is vaccination the birds with suitable vaccine protecting against the circulating virus therefor this study for production of TKPV vaccine. TKPV was isolated by inoculation of eleven day old embryonated chicken egg on chorioallantoic membrane (CAM) then the egg adapted TKPV was propagated on chicken embryo fibroblast (CEF) till adaptation at the 15th passage when titer was reaching to log 10 5.5 TCID 50/ ml . The adapted virus was transmitted to Vero cell line to produce the qualified vaccine. All quality control measures approved that the vaccine is ready to be used to control the TKPV disease in Egypt. C4L like gene expression was employed to evaluate TKPV virulence via Quantitative RT-PCR to ensure TKPV attenuation that begin from the 15th passage till complete attenuation at the 20th passage.

Keywords

• Turkey Pox Virus
• Quantitative RT-PCR