Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation

Natalie C. Bamford; François Le Mauff; Jaime C. Van Loon; Hanna Ostapska; Brendan D. Snarr; Yongzhen Zhang; Elena N. Kitova; John S. Klassen; Jeroen D. C. Codée; Donald C. Sheppard; P. Lynne Howell

doi:10.1038/s41467-020-16144-5

Nature Communications (May 2020)

Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation

Natalie C. Bamford,
François Le Mauff,
Jaime C. Van Loon,
Hanna Ostapska,
Brendan D. Snarr,
Yongzhen Zhang,
Elena N. Kitova,
John S. Klassen,
Jeroen D. C. Codée,
Donald C. Sheppard,
P. Lynne Howell

Affiliations

Natalie C. Bamford: Program in Molecular Medicine, The Hospital for Sick Children
François Le Mauff: Department of Microbiology and Immunology, Faculty of Medicine, McGill University
Jaime C. Van Loon: Program in Molecular Medicine, The Hospital for Sick Children
Hanna Ostapska: Department of Microbiology and Immunology, Faculty of Medicine, McGill University
Brendan D. Snarr: Department of Microbiology and Immunology, Faculty of Medicine, McGill University
Yongzhen Zhang: Leiden Institute of Chemistry, Leiden University
Elena N. Kitova: Alberta Glycomics Centre and Department of Chemistry, University of Alberta
John S. Klassen: Alberta Glycomics Centre and Department of Chemistry, University of Alberta
Jeroen D. C. Codée: Leiden Institute of Chemistry, Leiden University
Donald C. Sheppard: Department of Microbiology and Immunology, Faculty of Medicine, McGill University
P. Lynne Howell: Program in Molecular Medicine, The Hospital for Sick Children

DOI: https://doi.org/10.1038/s41467-020-16144-5
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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The exopolysaccharide galactosaminogalactan (GAG) is an important virulence factor of the fungal pathogen Aspergillus fumigatus. Here, the authors study an A. fumigatus enzyme that deacetylates GAG in a metal-dependent manner and constitutes a founding member of a new carbohydrate esterase family.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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