Characterization of polymeric nanoparticles for treatment of partial injury to the central nervous system
Ivan Lozić,
Richard V. Hartz,
Carole A. Bartlett,
Jeremy A. Shaw,
Michael Archer,
Priya S.R. Naidu,
Nicole M. Smith,
Sarah A. Dunlop,
K. Swaminathan Iyer,
Matt R. Kilburn,
Melinda Fitzgerald
Affiliations
Ivan Lozić
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia; Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Richard V. Hartz
Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Carole A. Bartlett
Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Jeremy A. Shaw
Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Michael Archer
Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Priya S.R. Naidu
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia; Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Nicole M. Smith
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia; Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Sarah A. Dunlop
Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
K. Swaminathan Iyer
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Matt R. Kilburn
Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia
Melinda Fitzgerald
Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia; Corresponding author.
Before using nanoparticles for therapeutic applications, it is necessary to comprehensively investigate nanoparticle effects, both in vitro and in vivo. In the associated research article [1] we generate multimodal polymeric nanoparticles functionalized with an antibody, that are designed to deliver an anti-oxidant to astrocytes. Here we provide additional data demonstrating the effects of the nanoparticle preparations on an indicator of oxidative stress in an immortalized Müller cell line in vitro. We provide data demonstrating the use of nanoscale secondary ion mass spectroscopy (NanoSIMS) to identify specific ions in bulk dried NP. NanoSIMS is also used to visualize 40Ca microdomains in the z dimension of optic nerve that has been subjected to a partial optic nerve transection. The associated article [1] describes the use of NanoSIMS to quantify 40Ca microdomains in optic nerve from animals treated with various nanoparticle preparations and provides further interpretation and discussion of the findings.
