Онкогематология (Oct 2016)
Results of external quality control study in flow cytometric acute lymphoblastic leukemia diagnostics
- A. M. Popov,
- T. U. Verzhbitskaya,
- E. E. Zueva,
- O. V. Anan’eva,
- E. V. Babenko,
- L. V. Baydun,
- E. A. Belyakova,
- E. V. Boyakova,
- O. G. Bortnikova,
- M. V. Gorchakova,
- L. Yu. Grivtsova,
- E. N. Grinkevich,
- Yu. V. Davydova,
- E. I. Zakhar’ko,
- O. I. Illarionova,
- N. M. Kaprano,
- E. A. Koroleva,
- S. A. Kochengina,
- E. G. Kuz’mina,
- E. A. Kustova,
- T. A. Makarova,
- Yu. V. Mirolyubova,
- O. E. Murashkina,
- T. Yu. Mushkarina,
- E. S. Nisheva,
- I. A. Novikova,
- E. Yu. Osipova,
- G. E. Pluzhnikova,
- M. E. Pochtar,
- N. V. Pronkina,
- E. V. Rusanova,
- E. B. Rybkina,
- O. V. Seliverstova,
- N. N. Tupitsin,
- G. I. Uleyskaya,
- N. T. Urazalieva,
- L. G. Fechina,
- O. V. Khoroshikh,
- O. E. Tsareva,
- L. A. Shchekina,
- S. A. Plyasunova,
- S. A. Lugovskaya
Affiliations
- A. M. Popov
- Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, Ministry of Health of Russia
- T. U. Verzhbitskaya
- Regional Children Clinical Hospital No 1
- E. E. Zueva
- Department of Molecular Biology, Faculty of Natural Sciences, Ariel University, Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia
- O. V. Anan’eva
- Tyumen State Medical University, Ministry of Health of Russia
- E. V. Babenko
- I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, R. M. Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantology
- L. V. Baydun
- Russian Children Clinical Hospital, Ministry of Health of Russia
- E. A. Belyakova
- North-Western State Medical University named after I.I. Mechnikov, Ministry of Health of Russia
- E. V. Boyakova
- Blood Transfusion Station of the Moscow Healthcare Department
- O. G. Bortnikova
- Regional Children Clinical Hospital
- M. V. Gorchakova
- Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia
- L. Yu. Grivtsova
- N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia
- E. N. Grinkevich
- Nizhnevartovsk Regional Children Clinical Hospital
- Yu. V. Davydova
- Nizhnevartovsk Regional Children Clinical Hospital
- E. I. Zakhar’ko
- Central Clinical Diagnostic Laboratory with a Group of Hemostasis Disorders, National Research Center for Hematology, Ministry of Health of Russia
- O. I. Illarionova
- Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, Ministry of Health of Russia
- N. M. Kaprano
- Scientific Clinical Laboratory of Immunophenotyping of Blood Cells and Bone Marrow, National Research Center for Hematology, Ministry of Health of Russia
- E. A. Koroleva
- Diagnostic Center of the Altai Region
- S. A. Kochengina
- Chelyabinsk Regional Children Clinical Hospital
- E. G. Kuz’mina
- A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center, Ministry of Health of Russia
- E. A. Kustova
- Research Center of Pediatrics and Pediatric Surgery
- T. A. Makarova
- Children City Hospital No 1
- Yu. V. Mirolyubova
- ФГБУ «Северо-Западный федеральный медицинский исследовательский центр» Минздрава России
- O. E. Murashkina
- Children Regional Clinical Hospital
- T. Yu. Mushkarina
- A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center, Ministry of Health of Russia
- E. S. Nisheva
- Children City Hospital No 1
- I. A. Novikova
- Rostov Research Institute of Oncology, Ministry of Health of Russia
- E. Yu. Osipova
- Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, Ministry of Health of Russia
- G. E. Pluzhnikova
- Regional Children Clinical Hospital
- M. E. Pochtar
- Department of Clinical Laboratory Diagnostics, Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia
- N. V. Pronkina
- Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
- E. V. Rusanova
- Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia
- E. B. Rybkina
- Central Clinical Diagnostic Laboratory with a Group of Hemostasis Disorders, National Research Center for Hematology, Ministry of Health of Russia
- O. V. Seliverstova
- Blood Transfusion Station of the Moscow Healthcare Department
- N. N. Tupitsin
- N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia
- G. I. Uleyskaya
- City Clinical Hospital No 31
- N. T. Urazalieva
- Research Center of Pediatrics and Pediatric Surgery
- L. G. Fechina
- Regional Children Clinical Hospital No 1
- O. V. Khoroshikh
- Irkutsk the Badge of Honor Order Regional Clinical Hospital
- O. E. Tsareva
- Saratov State Medical University named after V.I. Razumovsky, Ministry of Health of Russia
- L. A. Shchekina
- City Clinical Hospital No 31
- S. A. Plyasunova
- Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, Ministry of Health of Russia
- S. A. Lugovskaya
- Department of Clinical Laboratory Diagnostics, Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia
- DOI
- https://doi.org/10.17650/1818-8346-2016-11-3-68-75
- Journal volume & issue
-
Vol. 11,
no. 3
pp. 68 – 75
Abstract
Comparison of interpretation of acute lymphoblastic leukemia (ALL) flow cytometric diagnostics data was the aim of the study. Immunophenotyping data obtained from 10 patients with ALL were analysed separately in 26 laboratories from Russian Federation and Kazahstan. Results comparison showed four main type of discordance: B-lineage ALL diagnostics during heavy bone marrow regeneration, great variability of T-ALL interpretation, complexity of ambiguous lineage acute leukemia and, finally, very different report types, unique for each laboratory. All these problems are the serious obstacles for standardization of flow cytometric ALL diagnostics in multicenter setting. Continuation of similar QC rounds following by consecutive discussions with further development of consensus diagnostic algorithm could be the first step for standardization of ALL immunophenotyping in Russian Federation and CIS countries.
Keywords
