Drug Design, Development and Therapy (Dec 2021)
Lactobacillus fermentum CQPC08 Attenuates Exercise-Induced Fatigue in Mice Through Its Antioxidant Effects and Effective Intervention of Galactooligosaccharide
Abstract
Dong Liu,1,2 Da Chuan Liu,3 Hao Fan,4,5 Yu Wang6 1Development Chongqing University of Education, Chongqing, People’s Republic of China; 2Education Major in Physical Education, University of Perpetual Help System DALTA Las Pinas, Manila, Philippines; 3Student Affairs Department, Jiangmen Preschool Education College, Jiangmen, Guangdong, People’s Republic of China; 4School of Tourism and Service Management, Chongqing University of Education, Chongqing, People’s Republic of China; 5Cultural Industries and Cultural Policy, Yuan Ze University, Taoyuan, Taiwan; 6Orthopedics Department, General Hospital of Northern Theatre Command, Liaoning Province, People’s Republic of ChinaCorrespondence: Yu WangOrthopedics Department, General Hospital of Northern Theatre Command, No. 83 Wenhua Road, Shenhe District, Shenyang, Liaoning Province, 110016, People’s Republic of ChinaTel +86-18609886338Email [email protected]: The purpose of this study is to study the antioxidant effect of Lactobacillus fermentum CQPC08 (CQPC08) on exercise-induced fatigue, and the beneficial intervention of GOS on CQPC08.Methods: We use the treadmill to establish a fatigue model caused by exercise, and perform drug treatment after exercise. We tested the exhaustive exercise time of mice; investigated the changes of mice body weight, liver index, histopathology, serum biochemical indicators and mRNA expression levels of oxidative and inflammation-related genes; and assessed the potential fatigue inhibitory effect of CQPC08, and the anti-oxidation effect of the combination of GOS and CQPC08.Results: The results suggest that CQPC08 and combination with GOS reduces fatigue-induced oxidative damage of the liver, and it decreases blood urea nitrogen (BUN), lactic acid (LA), glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), malonaldehyde (MDA), inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in serum. Higher levels of serum catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were found. Treatment with the CQPC08 and combination with GOS correlates with lower relative mRNA expression levels of neuronal NOS (nNOS), iNOS, and TNF-α, and with higher mRNA expression levels of catalase and copper/zinc (Cu/Zn) and manganese (Mn) SOD enzymes in the liver and muscles.Conclusion: These results suggest that CQPC08 can resolve exercise-induced fatigue by improving antioxidant ability in mice, and the combination of GOS and CQPC08 enhances this ability of CQPC08.Keywords: Lactobacillus fermentum, exercise-induced fatigue, antioxidant, GOS
