Cell suspension culture of Amsonia tabernaemontana Walter: growth, organogenesis and alkaloid production

Abstract

The paper discusses the growth of cell suspension cultures of Amsonia tabernaemontana Walter established from callus of hypocotyl origin. The cell number and growth increment were determined. Cellular aggregates developed well in the Wood and Braun (WB) medium with 1 mg/l NAA and 0.5 mg/l kinetin (growth increment 712.4). When the aggregates were cultured on WB media without NAA and kinetin or with 0.02 mg/l kinetin and 3 mg/l IAA, Toots developed an the aggregates. Examiination of the roots and cell suspensions indicates that the Toots are richer in alkaloids than the callus and cell suspensions.