THE PRIMER EXTENSION TECHNIQUE FOR THE POLYMORPHISM DETECTION AT OVINE PRN-P LOCUS

Abstract

Scrapie is a prionic illness with endemic character in many parts of the glob, and the control measures is difficult to apply because of the long incubation period, the lack of the preclinical manifestation and the existing tests for diagnostic in living animals. The Ppn-p locus is polymorphic with known variability at codon 136, 154, 171, which are associated with different sensibility in experimental and natural spongiform encephalopaties. General the possible combinations of the 5 amino acids encoded by the 3 different codons will determine the existence of 15 possible genotypes. To put in evidence those polymorphisms at the ovine Prn-p locus, several methods are developed but the most accurate assay is the direct sequencing of the gene and the primer extension technique. The purpose of this study was to determine the genotypes at Prp locus in 123 male of Tsurcana breed, Hateg ecotype, using primer extension technique (ABI 3130xl Genetic Analyzer) and to establish the risk groups of the susceptibility at scrapie disease.

Keywords

• scrapie
• prion
• prnp locus
• primer extension
• tse