Two-dimensional (2D) transition-metal dichalcogenides (TMDCs) quantum dots (QDs) are the vanguard due to their unique properties. In this work, WSe2 QDs were fabricated via one step ultrasonic probe sonication. Excitation wavelength dependent photoluminescence (PL) is observed from WSe2 QDs. Room-temperature lasing emission which benefits from 3.7 times enhancement of PL intensity by thermal treatment at ~470 nm was achieved with an excitation threshold value of ~3.5 kW/cm2 in a Fabry–Perot laser cavity. To the best of our knowledge, this is the first demonstration of lasing emission from TMDCs QDs. This indicates that TMDCs QDs are a superior candidate as a new type of laser gain medium.