Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPCL Technique: a Preliminary Report

Abstract

A significant population of ultrashort (50–150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL−1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.

Keywords

• retinoblastoma
• blood plasma
• cfdna
• ssdna
• hplc