Reusability of immobilized β-glucosidase on sodium alginate-coated magnetic nanoparticles and high productivity applications

Fawze Alnadari; Yemin Xue; Nouf H. Alsubhi; Soha A. Alamoudi; Afaf S. Alwabli; Diana A. Al-Quwaie; Yahya Saud Hamed; Mustapha Muhammad Nasiru; Alia A.M. Ebrahim; Mohamed T. El-Saadony; Fei Pan

Journal of Saudi Chemical Society (Sep 2022)

Reusability of immobilized β-glucosidase on sodium alginate-coated magnetic nanoparticles and high productivity applications

Fawze Alnadari,
Yemin Xue,
Nouf H. Alsubhi,
Soha A. Alamoudi,
Afaf S. Alwabli,
Diana A. Al-Quwaie,
Yahya Saud Hamed,
Mustapha Muhammad Nasiru,
Alia A.M. Ebrahim,
Mohamed T. El-Saadony,
Fei Pan

Affiliations

Fawze Alnadari: Department of Food Science and Engineering, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, PR China; Department of Food Science and Nutrition, Faculty of Agriculture, Food and Environment, Sana’a University, Sana’a, Yemen; Department of Food Science and Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
Yemin Xue: Department of Food Science and Engineering, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, PR China
Nouf H. Alsubhi: Biological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia
Soha A. Alamoudi: Biological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia
Afaf S. Alwabli: Biological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia
Diana A. Al-Quwaie: Biological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia
Yahya Saud Hamed: Department of Food Technology, Faculty of Agriculture, Suez Canal University, Ismailia 41522 Egypt
Mustapha Muhammad Nasiru: Department of Food Science and Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
Alia A.M. Ebrahim: School of Life Science, Nanjing Normal University, Nanjing, Jiangsu, PR China
Mohamed T. El-Saadony: Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt; Corresponding author.
Fei Pan: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, PR China

Journal volume & issue: Vol. 26, no. 5
p. 101517

Abstract

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The purpose of this study is to detect the impact of the covalent immobilization of β-glucosidase (β-Glu) from Thermatoga maritima on sodium alginate-based magnetically recyclable iron nanoparticles (SA-Fe3O4 MNPs) for boosting robustness, facile retrieval, and recycling of enzyme. We developed a novel green method to amend the transformation of lactose to galacto-oligosaccharides (GOS). The interaction of the Immobilized β-Glu and SA-Fe3O4 MNPs were characterized by SDS-PAGE, Transmission electron microscope (TEM) and FT-IR spectroscopy. After immobilization, the ideal pH maintained 7.0, the optimum temperature (temp.) increased to 85 °C and deceptive Km and Vmax raised by 65 and 41%, respectively. Immobilization provided low activation energy and enhanced pH and heat stability. The SA-Fe3O4 MNPs maintained the effectiveness of enzyme by 39.52% up to the 6th cycle of reuse and the hydrolysis power exceed 28.13% of lactose to GOS. Conclusively, thermal stability, improved pH, and excellent reusability over β-Glu make SA-Fe3O4 MNPs a viable option for long-term GOS production as well as other industrial uses.

Published in Journal of Saudi Chemical Society

ISSN: 1319-6103 (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Science: Chemistry
Website: http://www.journals.elsevier.com/journal-of-saudi-chemical-society/

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