The Arrangement of Microtubules in Embryos Derived from Mice Young, Old and Reconstructed Oocytes

Abstract

Objective: To study the structure and distribution of microtubules in embryos derivedfrom young, old and reconstructed oocytes.Materials and Methods: Embryos obtained from old (50 embryos), young(50 embryos) and reconstructed oocytes (10 embryos) were studied byimmunocytochemistry. The microtubule structures of the embryos were studied byusing fluroscent microscopy with FITC-PI filter and polyclonal antibody against alfatubulin.Results: The spindle structure of MII young oocyte and the obtained embryos werenormal with the suitable condensation. There was no contact between chromosomeand spindle in old Oocytes as well as the obtained embryos, in addition, the spindlewas extended in old group. In reconstructed embryos, thin and scattered filamentswere observed.Conclusion: This study reveals that the arrangement of microtubules inreconstructed embryos was caused by repeating of injection and oocytemanipulation. Also, interactions between karyoblast, cytoplasm and microtubulsmay not be suitable. This may be caused by low fertilization in these oocytes.

Keywords

• Microtubules
• Mitotic Spindle
• Reconstructed Oocytes