Molecular detection of Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, and Burkholderia glumae in infected rice seeds and leaves
Wen Lu,
Luqi Pan,
Haijun Zhao,
Yulin Jia,
Yanli Wang,
Xiaoping Yu,
Xueyan Wang
Affiliations
Wen Lu
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Science, China Jiliang University, Hangzhou 310018, China
Luqi Pan
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Science, China Jiliang University, Hangzhou 310018, China
Haijun Zhao
Institute of Nuclear-Agricultural Science, Zhejiang University, Hangzhou 310029, China
Yulin Jia
United States Department of Agriculture, Agricultural Research Service, Dale Bumpers National Rice Research Center (USDA-ARS DB NRRC), Stuttgart, AR, USA
Yanli Wang
Institute of Plant Protection and Microbe, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Xiaoping Yu
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Science, China Jiliang University, Hangzhou 310018, China
Xueyan Wang
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Science, China Jiliang University, Hangzhou 310018, China
The polymerase chain reaction (PCR) is particularly useful for plant pathogen detection. In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, and Burkholderia glumae. The unique PCR primer sets were designed from portions of a putative glycosyltransferase gene of X. oryzae pv. oryzae, an AvrRxo gene of X. oryzae pv. oryzicola, and an internal transcribed spacer (ITS) sequence of B. glumae. Using a multiplex PCR assay, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected in one PCR reaction that contained the newly developed primer set mix. Using SYBR Green real-time PCR assays, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected at 1, 1, and 10 fg μL− 1, respectively. These newly designed molecular assays are sensitive and could be reliable tools for pathogen detection and disease forecasting.
