Veterinary Sciences (Dec 2024)

A Transcriptome Approach Evaluating the Effects of Atractylenolide I on the Secretion of Estradiol and Progesterone in Feline Ovarian Granulosa Cells

  • Yuli Guo,
  • Junping Liu,
  • Shuangyi Zhang,
  • Di Sun,
  • Zhiying Dong,
  • Jinshan Cao

DOI
https://doi.org/10.3390/vetsci11120663
Journal volume & issue
Vol. 11, no. 12
p. 663

Abstract

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Atractylodes macrocephala Koidz (AMK) as an oriental medicine has been used in the treatment of threatened abortion. Atractylenolide I (AT-I) is one of the major bioactive components of AMK. This study aimed to investigate the effect of AT-I on the secretion of estradiol (E2) and progesterone (P4) in feline ovarian granulosa cells (FOGCs), which is necessary for pregnancy. At first, the proliferation of FOGCs after AT-I treatment was measured by CCK-8. Then, the synthesis of E2 and P4 were measured by ELISA. Lastly, transcriptome sequencing was used to detect the DEGs in the FOGCs, and RNA-seq results were verified by RT-qPCR and biochemical verification. It was found that AT-I could promote proliferation and the secretion of E2 and P4 in FOGCs; after AT-I treatment, 137 significant DEGs were observed, out of which 49 were up-regulated and 88 down-regulated. The DEGs revealed significant enrichment of 52 GO terms throughout the differentiation process (p p 2 and P4, which might be achieved by activation of cholesterol metabolism.

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