Acta Dermato-Venereologica (Nov 2020)

Protein and mRNA Expression Levels of Interleukin-17A, -17F and -22 in Blood and Skin Samples of Patients with Mycosis Fungoides

  • Despoina Papathemeli,
  • Aikaterini Patsatsi,
  • Dimitrios Papanastassiou,
  • Triantafyllia Koletsa,
  • Thomas Papathemelis,
  • Chrysostomos Avgeros,
  • Olga Pikou,
  • Elizabeth Lazaridou,
  • Elisavet Georgiou

DOI
https://doi.org/10.2340/00015555-3688
Journal volume & issue
Vol. 100, no. 18
p. adv00326

Abstract

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This study investigated the expression of interleukin (IL)-17A, -17F and -22 in mycosis fungoides. Blood samples were collected from 50 patients with mycosis fungoides and 50 healthy controls. Skin samples were obtained from 26 patients with mycosis fungoides and 5 healthy controls. Protein levels of IL-17A, -17F and -22 were measured in serum by multiplex enzyme-linked immunosorbent assay, and mRNA expression levels were measured in blood and skin samples by real-time quantitative reverse transcription PCR. Both IL-17A and IL-17F mRNA expression levels were significantly lower in blood of patients with mycosis fungoides in comparison with healthy controls. IL-22 serum levels and expression levels of IL-22 mRNA in skin tissue, were significantly increased in patients with mycosis fungoides in comparison with healthy controls. These results suggest that low levels of IL-17A and IL-17F in mycosis fungoides may be connected to impaired immune surveillance contributing to tumourigenesis. Upregulation of IL-22 may play a role in the establishment of the tumour microenvironment in mycosis fungoides.

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