Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries

Abstract

Highlights 1. Key sites were proved to promote low-temperature catalytic efficiency of xylanase. 2. Thermostable mutant with improved catalytic efficiency at 40 °C was obtained. 3. Synergistic hydrolysis was dramatically improved between the mutant and cellulase. 4. Agricultural wastes were converted into fermentable sugars by enzymolysis.

Keywords

• GH10 xylanase
• Low-temperature catalytic performance
• Protein engineering
• Synergism
• Biomass degradation