Chitosan-collagen-cerium hydroxyapatite nanocomposites for In-vitro gentamicin drug delivery and antibacterial properties

Abstract

The controlled release of antibiotics is crucial to improving antimicrobial efficacy, reducing the risk of bacterial resistance, and ensuring a localized therapeutic effect. In this work, In-vitro Gentamicin release was studied using fluorescence chitosan collagen-cerium hydroxyapatite nanocomposites. Cerium-hydroxyapatite nanoparticles were synthesized using the hydrothermal method, and the nanocomposites were prepared by mixing chitosan-collagen-cerium hydroxyapatite at different weight ratios. Structural characterization was conducted using scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, and fluorescence microscopy. Ultraviolet–visible spectroscopy (UV–Vis) was used to quantify the release of gentamicin in simulated body fluid. Results showed that hydroxyapatite releases 90 % of gentamicin in the first 10 min, and the Chitosan-collagen-cerium hydroxyapatite nanocomposites release 80 % of gentamicin after 2 h. The antibacterial activity was studied against Escherichia coli (E. coli) at different time intervals. These nanocomposites can potentially improve the performance of biomedical applications.

Keywords

• Gentamicin
• Chitosan
• Collagen
• Hydroxyapatite
• Nanocomposites
• Antibacterial properties