Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems

Abstract

Summary: Microscopy-based analysis of protein accumulation at a given subcellular location in real time provides invaluable insights into the function of a protein in a specific process. Here, we describe a detailed protocol for determining protein accumulation kinetics at the division site in the budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe. This protocol can be adapted for the analysis of any protein involved in any process as long as the protein is localized to a discrete region of the cell.For complete details on the use and execution of this protocol, please refer to Okada et al. (2021) and Okada et al. (2019).

Keywords

• Cell Biology
• Genetics
• Microscopy
• Model Organisms