Determination of bisphenol a in baby bottles and drinking containers by high liquid chromatography

Abstract

Bisphenol A is a monomer used primarily in the production of polycarbonate plastics and epoxy resins. Polycarbonate plastic is used in a wide variety of digital media products, electrical and electronic equipment, sport safety equipment, reusable food and drink containers, etc. Epoxy resins are used in engineering applications, paints and adhesives and also in a variety of protective coatings in metal cans for foods, bottle tops and water supply pipes. The content of BPA was evaluated in 16 samples (6 baby bottles and 10 drinking containers - can) collected during 2018/2019, of which three baby bottles and 8 cans were collected in 2018, while three baby bottles and 2 cans in 2019. A simple HPLC-FLD analytical method was validated for the quantitation of BPA from baby feeding bottles and cans. The separation was performed on a C18 column. Good linearity was obtained over the concentration range of 0.3-6.0 μg/mL with the regression coefficient (R2) of 0.9998. The limit of detection (LOD) and limit of quantification (LOQ) were 0.01 and 0.03 μg/mL, respectively. The repeatability of the method (%RSD) was between 4 and 6%, while the recovery ranged from 104.3 to 109.7%. The extraction of BPA was done in accordance with SRPS EN 13130-1:2008. The methods were applied to determine BPA release from baby bottles, performing repeated procedures according to EU and national regulations (SRPS CEN/TS 13130-13 (2008)). The conformity was evaluated according to National and EU requirements. The results show that Bisphenol-A was not detectable in any of the analysed samples. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR31038]

Keywords

• bisphenol a
• baby bottles
• drink containers