Nature Communications (May 2017)
Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A–SF3b complex
- Teng Teng,
- Jennifer HC Tsai,
- Xiaoling Puyang,
- Michael Seiler,
- Shouyong Peng,
- Sudeep Prajapati,
- Daniel Aird,
- Silvia Buonamici,
- Benjamin Caleb,
- Betty Chan,
- Laura Corson,
- Jacob Feala,
- Peter Fekkes,
- Baudouin Gerard,
- Craig Karr,
- Manav Korpal,
- Xiang Liu,
- Jason T. Lowe,
- Yoshiharu Mizui,
- James Palacino,
- Eunice Park,
- Peter G. Smith,
- Vanitha Subramanian,
- Zhenhua Jeremy Wu,
- Jian Zou,
- Lihua Yu,
- Agustin Chicas,
- Markus Warmuth,
- Nicholas Larsen,
- Ping Zhu
Affiliations
- Teng Teng
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Jennifer HC Tsai
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Xiaoling Puyang
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Michael Seiler
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Shouyong Peng
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Sudeep Prajapati
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Daniel Aird
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Silvia Buonamici
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Benjamin Caleb
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Betty Chan
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Laura Corson
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Jacob Feala
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Peter Fekkes
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Baudouin Gerard
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Craig Karr
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Manav Korpal
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Xiang Liu
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Jason T. Lowe
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Yoshiharu Mizui
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- James Palacino
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Eunice Park
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Peter G. Smith
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Vanitha Subramanian
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Zhenhua Jeremy Wu
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Jian Zou
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Lihua Yu
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Agustin Chicas
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Markus Warmuth
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Nicholas Larsen
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- Ping Zhu
- H3 Biomedicine Inc., 300 Technology Sq, 5th Floor, Cambridge, Massachusetts 02139, USA
- DOI
- https://doi.org/10.1038/ncomms15522
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 16
Abstract
A number of natural occurring small-molecule splicing modulators are known. Here, the authors combine chemogenomic, structural and biochemical methods and show that these compounds also target the spliceosome-associated protein PHF5A and propose a potential modulator binding site in the PHF5A–SF3B1 complex.
