بیولوژی کاربردی (Jun 2020)
The Frequency of of class 1, 2, and 3 Integrons and Extended- spectrum Beta-Lactamase Genes of bla-CTX-M‚ bla-SHV‚ and bla-TEM in Gram-negative Bacilli Isolated from Bandar Abbas Pediatric Hospital, Iran-2016
Abstract
Background and Objective:Gram-negative bacilli are important hospital pathogens with an increasing prevalence of broad-spectrum beta-lactamase genes and integrons. Therefore, identification of these antibiotic resistance genes is essential to prevent the spread of resistant strains.The aim of this study was to determine the frequency of class 1, 2 and 3 integrons and bla-CTX-M, bla-SHV and bla-TEM broad-spectrum beta-lactamases genes in Gram-negative bacilli isolated from Bandar Abbas Pediatric Hospital. Materials and Methods: Sixty Gram-negative bacilli strains were isolated from clinical specimens and identified by biochemical tests and their antibiotic resistance patterns were determined by Disk Diffusion method. Multiplex PCR was used for detection of class 1, 2 and 3 integronsand PCR wasperformed to identify the bla-CTX-M, bla-SHV and bla-TEM family’s genes, respectively. Results:The most frequent strains belonged to Escherichia coli 70% and the highest resistance and sensitivity were Sulfomethoxazole 68% and Gentamicin 75% respectively.Of the 60 strains isolated, 61.7% and 26.7% had Class I and 2 integron genes, respectively, whereas no class 3 integron gene was detected in any of the isolates. PCR results showed that blaCTX-M, blaSHV and blaTEM family genes were 66.7%, 10% and 31. 7% strains, respectively. Conclusion: In this study, there was a significant correlation (p < 0.05) between the presence of class 1 and 2 integrons, bla-CTX-M, bla-SHV and bla-TEMand antibiotic resistance. Therefore, determination of antibiotic resistance genes and the use of appropriate therapeutic methods based on antibiogram pattern determination of the strains are also suggested.
