Nature Communications (Aug 2021)

Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids

  • Lixia Fang,
  • Jie Fan,
  • Shulei Luo,
  • Yaru Chen,
  • Congya Wang,
  • Yingxiu Cao,
  • Hao Song

DOI
https://doi.org/10.1038/s41467-021-25243-w
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 13

Abstract

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Identification of gene targets is one of the major challenges to construct superior microbial cell factory for chemical synthesis. Here, the authors employ CRISPRi and omics analyses for genome-scale target genes identification for high-titer production of free fatty acids in E. coli.