Journal of Cardiothoracic Surgery (Nov 2021)

MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

  • Jiangwei Hu,
  • Xinqin Xiang,
  • Wei Guan,
  • Weihua Lou,
  • Junming He,
  • Jian Chen,
  • Yin Fu,
  • Guoliang Lou

DOI
https://doi.org/10.1186/s13019-021-01698-2
Journal volume & issue
Vol. 16, no. 1
pp. 1 – 8

Abstract

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Abstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. Results Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. Conclusion By targeting CDCA4, miR-497-5p restrained development of LUSC.

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