Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells

Pin-Chao Liao; Emily J. Yang; Liza A. Pon

STAR Protocols (Dec 2020)

Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells

Pin-Chao Liao,
Emily J. Yang,
Liza A. Pon

Affiliations

Pin-Chao Liao: Department of Pathology and Cell Biology, Columbia University, New York, NY, USA
Emily J. Yang: Department of Pathology and Cell Biology, Columbia University, New York, NY, USA
Liza A. Pon: Department of Pathology and Cell Biology, Columbia University, New York, NY, USA; Corresponding author

Journal volume & issue: Vol. 1, no. 3
p. 100160

Abstract

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Summary: The redox state of mitochondria is one indicator of the functional state of the organelles. Mitochondria are also the primary endogenous source of reactive oxygen species (ROS). Therefore, the redox state of the organelles also reflects their function in ROS production. Here, we provide step-by-step protocols for live-cell imaging and quantification of mitochondrial redox state using the genetically encoded fluorescent biosensor, mitochondria-targeted redox sensing GFP (mito-roGFP), and mitochondrial ROS using the membrane-permeant small molecule dihydroethidium (DHE) in budding yeast cells.For complete details on the use and execution of this protocol, please refer to Liao et al. (2020c).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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