Development of the A&GBE base editor for efficiently generating abundant genetic variation in rice
Wenhui Zhang,
Yaqi Xu,
Jingxuan Cui,
Yingshuang Xue,
Lijie Zhang,
Wenjuan Wu,
Chong Wang,
Hui Zhang
Affiliations
Wenhui Zhang
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Yaqi Xu
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Jingxuan Cui
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Yingshuang Xue
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Lijie Zhang
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Wenjuan Wu
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Chong Wang
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Hui Zhang
Corresponding author.; Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
We have developed a dual base editor, rA&GBE, by fusing adenine and glycosylase base editors. It can induce up to eight types of mutations in T0-generation rice, including single-base conversion, simultaneous multiple-base conversions, and InDels, using a single guide RNA. A-to-G and C-to-G/T conversions occur simultaneously on the same DNA strand. The rA&GBE system may prove useful for crop improvement and in planta direct evolution.
