SPECTROPHOTOMETRIC AND LUMINESCENCE DETERMINATION OF ABIRATERONE ACETATE

Abstract

The methods of the spectrophotometric (SP) and fluorimetric (FL) determination of a new anticancer drug, abiraterone acetate (AA) have been proposed. SP method is based on measurement of light absorbance of ethanol solution of AA at a wavelength 253 nm. The calibration graph (CG) is linear in the concentration range 1.0–30.0 AA μg/ml, the limit of detection (LOD) is 0.35 μg/ml.Two versions of FL determination of AA: on its native luminescence (λex=253 nm; λlum=350 nm) and on quenching sensitized luminescence of the terbium(III) complex by abiraterone acetate have been developed. CG in the case of native fluorescence is linear in the range of 0.05–7.0 μg/ml, Stern-Folmer calibration curve is linear in the interval of concentrations of AA 0.5– 70 μg/ml. LOD of both fluorescent methods is 0.017 μg/ml.

Keywords

• спектрофотометрія, флуориметрія, сенсибілізована люмінесценція тербію(III), абіратерону ацетат