Nature Communications (Aug 2021)
A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA
- Mohsen Mohammadniaei,
- Ming Zhang,
- Jon Ashley,
- Ulf Bech Christensen,
- Lennart Jan Friis-Hansen,
- Rasmus Gregersen,
- Jan Gorm Lisby,
- Thomas Lars Benfield,
- Finn Erland Nielsen,
- Jens Henning Rasmussen,
- Ellen Bøtker Pedersen,
- Anne Christine Rye Olinger,
- Lærke Tørring Kolding,
- Maryam Naseri,
- Tao Zheng,
- Wentao Wang,
- Jan Gorodkin,
- Yi Sun
Affiliations
- Mohsen Mohammadniaei
- Department of Health Technology, Technical University of Denmark
- Ming Zhang
- Department of Health Technology, Technical University of Denmark
- Jon Ashley
- Department of Health Technology, Technical University of Denmark
- Ulf Bech Christensen
- PentaBase A/S
- Lennart Jan Friis-Hansen
- Department of Clinical Biochemistry, Copenhagen University Hospital - Bispebjerg and Frederiksberg
- Rasmus Gregersen
- Department of Emergency Medicine, Copenhagen University Hospital - Bispebjerg and Frederiksberg
- Jan Gorm Lisby
- Department of Clinical Microbiology 445, Copenhagen University Hospital - Amager and Hvidovre
- Thomas Lars Benfield
- Department of Infectious Diseases, Copenhagen University Hospital - Amager and Hvidovre
- Finn Erland Nielsen
- Department of Emergency Medicine, Copenhagen University Hospital - Bispebjerg and Frederiksberg
- Jens Henning Rasmussen
- Department of Emergency Medicine, Copenhagen University Hospital - Bispebjerg and Frederiksberg
- Ellen Bøtker Pedersen
- Department of Occupational and Environmental Medicine, Copenhagen University Hospital - Bispebjerg and Frederiksberg
- Anne Christine Rye Olinger
- Department of Occupational and Environmental Medicine, Copenhagen University Hospital - Bispebjerg and Frederiksberg
- Lærke Tørring Kolding
- Department of Occupational and Environmental Medicine, Copenhagen University Hospital - Bispebjerg and Frederiksberg
- Maryam Naseri
- Department of Health Technology, Technical University of Denmark
- Tao Zheng
- Department of Health Technology, Technical University of Denmark
- Wentao Wang
- Department of Health Technology, Technical University of Denmark
- Jan Gorodkin
- Center for non-coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Yi Sun
- Department of Health Technology, Technical University of Denmark
- DOI
- https://doi.org/10.1038/s41467-021-25387-9
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 12
Abstract
The reliance on enzymes in SARS-CoV-2 RNA detection imposes limits on transport and storage conditions. Here the authors use non-enzymatic isothermal amplification to detect RNA with no need for reverse transcription.
