FEATURES OF PROLIFERATIVE ACTIVITY OF CERVICAL TISSUE IN THE PRESENCE OF ALCOHOL DEPENDENCE

Abstract

Alcohol consumption is one of the main risk factors for disease. Alcohol abuse is associated with the risk of genital injury due to unprotected sex, multiple sexual partners. The risk of cervical transformation in alcohol abusers remains is controversial. The aim of our work was to identify the features of the proliferative activity of cervical tissue in the presence of alcohol dependence. Object and methods. We investigated 50 sectional cases of women who died from chronic alcoholism. The age of women ranged from 24 to 46 years, with an average of 32.7 years. The cervix was examined histologically, immunohistochemically (with monoclonal antibodies to Ki-67), a morphometric study was performed (the thickness of the epithelial layer of the cervix was determined). Expression was assessed using a semi-quantitative scale. Results and conclusions. In the study group, a significant increase in the specific volume of cases with dysplastic changes was revealed. We revealed preserved anisomorphism and stratification of the surface and intermediate layers, characteristic of mild dysplasia, focal basal cell hyperactivity with an increase in the nuclear-cytoplasmic ratio, which is a sign of increased proliferative activity. It was found that women who abuse alcohol more often have cervical dysplasia. Exactly the more frequent cases of cervical dysplasia that can be one of the possible reasons for the increase in the thickness of the epithelium; the thickness of the epithelial layer of the cervix in this group is thickened to 697.8±47.88 x 10-6 m (in the comparison group 527.23±44.37 x 10-6 m, (p=0.038)). There is an increase in proliferative activity (the number of cells expressing Ki67) to 30.24±6.75% in the group with alcoholism (in the comparison group, 13.28±6.13% (p=0.043)). A strong direct correlation was established between the Ki 67 expression level and the thickness of the epithelial layer of the cervix (r=0.774, p<0.01). These results indicate the need to include women who abuse alcohol in the risk group for developing neoplastic transformation of the cervix.

Keywords

• cervix
• alcoholism
• epithelium
• histology
• proliferation
• ki67.