Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish
Alanazi Fehaid,
Elderdery Abozer Y.,
Alzahrani Badr,
Alzerwi Nasser A. N.,
Althobiti Maryam Musleh,
Rayzah Musaed,
Alanazi Abdulaziz Suailem,
Kuriri Fahd A.,
Idrees Bandar,
Alenazy Fawaz O.,
Alsultan Afnan,
Rayzah Fares,
Baksh Yaser,
Subbiah Suresh Kumar,
Mok Pooi Ling
Affiliations
Alanazi Fehaid
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences-AlQurayyat, Jouf University, Sakaka, Saudi Arabia
Elderdery Abozer Y.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia
Alzahrani Badr
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia
Alzerwi Nasser A. N.
Department of Surgery, College of Medicine, Majmaah University, P. O. Box 66, Al-Majmaah 11952, Riyadh, Kingdom of Saudi Arabia
Althobiti Maryam Musleh
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra, Riyadh Province 15572, Saudi Arabia
Rayzah Musaed
Department of Surgery, College of Medicine, Majmaah University, P. O. Box 66, Al-Majmaah 11952, Riyadh, Kingdom of Saudi Arabia
Alanazi Abdulaziz Suailem
Pathology and Clinical Laboratory Medicine Administration - Flow Cytometry, King Fahad Medical City, Riyadh, Saudi Arabia
Kuriri Fahd A.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra, Riyadh Province 15572, Saudi Arabia
Idrees Bandar
Department of Surgery, Prince Sultan Military Medical City in Riyadh, Makkah Al Mukarramah Rd, As Sulimaniyah, Saudi Arabia
Alenazy Fawaz O.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia
Alsultan Afnan
Department of Surgery, King Saud Medical City, Riyadh12746, Saudi Arabia
Rayzah Fares
Department of Surgery, Aseer Central Hospital, Abha62523, Saudi Arabia
Baksh Yaser
Department of Surgery, Iman General Hospital, Riyadh12684, Saudi Arabia
Subbiah Suresh Kumar
Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai, India
Mok Pooi Ling
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400UPM Serdang, Selangor, Malaysia
In this study, chlorogenic acid nanoparticles encapsulated in Pluronic F127 polymer were synthesized and characterized to determine if they could treat human liver cancer. The nanoparticles were synthesized using standard procedures and characterized using physical and biological techniques such as X-ray diffraction, Fourier transform infrared spectroscopy, UV-Vis, dynamic light scattering, Photoluminescence, scanning electron microscopy, and transmission electron microscopy. The anticancer effects were assessed using MTT analysis, acridine orange/ethidium bromide, reactive oxygen species (ROS), COMET assay, annexin-V/FITC, cell cycle analysis, and expression of marker genes against HepG2 cell lines. The results showed significant cytotoxicity, apoptosis induction, and increased ROS production in treated cells compared to control cells. The nanoparticles also activated the apoptotic cascade and regulated the PI3K/AKT/mTOR pathways. The nanocomposites exhibited unique characteristics such as anticancer efficacy in vitro. Further research was conducted using zebrafish to model hematological parameters, liver enzymes, and histopathology to study effectiveness. Green-synthesized Pluronic F127–chlorogenic acid nanoparticles can be considered potential cancer therapy agents.
