Preparation and characterization of two types of covalently immobilized amyloglucosidase

Abstract

Amyloglucosidase from A. niger was covalently immobilized onto poly (GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined.

Keywords

• glucoamylase
• poly(GMA-co-EGDMA)
• immobilization
• periodate
• starch