Molecular and seroepidemiological investigation of Сoxiella burnetii and spotted fever group rickettsiae in the southern region of Kazakhstan

Yuliya V. Perfilyeva; Zhanna A. Berdygulova; Akzhigit S. Mashzhan; Andrey V. Zhigailov; Yekaterina O. Ostapchuk; Dinara A. Naizabayeva; Alena S. Cherusheva; Akerke O. Bissenbay; Saltanat A. Kuatbekova; Nurshat Abdolla; Anna S. Nizkorodova; Maxim V. Kulemin; Zhanna Zh. Shapiyeva; Zaure Z. Sayakova; Anastassiya V. Perfilyeva; Ilyas A. Akhmetollayev; Elina R. Maltseva; Yuriy A. Skiba; Seidigapbar M. Mamadaliyev; Andrey M. Dmitrovskiy

Ticks and Tick-Borne Diseases (Nov 2023)

Molecular and seroepidemiological investigation of Сoxiella burnetii and spotted fever group rickettsiae in the southern region of Kazakhstan

Yuliya V. Perfilyeva,
Zhanna A. Berdygulova,
Akzhigit S. Mashzhan,
Andrey V. Zhigailov,
Yekaterina O. Ostapchuk,
Dinara A. Naizabayeva,
Alena S. Cherusheva,
Akerke O. Bissenbay,
Saltanat A. Kuatbekova,
Nurshat Abdolla,
Anna S. Nizkorodova,
Maxim V. Kulemin,
Zhanna Zh. Shapiyeva,
Zaure Z. Sayakova,
Anastassiya V. Perfilyeva,
Ilyas A. Akhmetollayev,
Elina R. Maltseva,
Yuriy A. Skiba,
Seidigapbar M. Mamadaliyev,
Andrey M. Dmitrovskiy

Affiliations

Yuliya V. Perfilyeva: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86 Dosmukhamedov St., Almaty 050012, Kazakhstan
Zhanna A. Berdygulova: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; Al-Farabi Kazakh National University, 71 Al-Farabi Avenue, Almaty 050040, Kazakhstan; Corresponding authors at: Almaty Branch of the National Center for Biotechnology, At Central Reference Laboratory, 14 Zhahanger St, Almaty 050054, Kazakhstan.
Akzhigit S. Mashzhan: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; Al-Farabi Kazakh National University, 71 Al-Farabi Avenue, Almaty 050040, Kazakhstan; Corresponding authors at: Almaty Branch of the National Center for Biotechnology, At Central Reference Laboratory, 14 Zhahanger St, Almaty 050054, Kazakhstan.
Andrey V. Zhigailov: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86 Dosmukhamedov St., Almaty 050012, Kazakhstan
Yekaterina O. Ostapchuk: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86 Dosmukhamedov St., Almaty 050012, Kazakhstan
Dinara A. Naizabayeva: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; Al-Farabi Kazakh National University, 71 Al-Farabi Avenue, Almaty 050040, Kazakhstan
Alena S. Cherusheva: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan
Akerke O. Bissenbay: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; Al-Farabi Kazakh National University, 71 Al-Farabi Avenue, Almaty 050040, Kazakhstan
Saltanat A. Kuatbekova: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan
Nurshat Abdolla: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86 Dosmukhamedov St., Almaty 050012, Kazakhstan
Anna S. Nizkorodova: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86 Dosmukhamedov St., Almaty 050012, Kazakhstan
Maxim V. Kulemin: Anti-Plague Station, 114 Dulati St., Shymkent 160013, Kazakhstan
Zhanna Zh. Shapiyeva: Scientific Practical Center of Sanitary-Epidemiological Expertise and Monitoring, 84 Auezov St., Almaty 050008, Kazakhstan
Zaure Z. Sayakova: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M. Aikimbayev National Scientific Center for Especially Dangerous Infections, 14 Zhahanger St., Almaty 050054, Kazakhstan
Anastassiya V. Perfilyeva: Institute of Genetics and Cytology, 93 Al-Farabi Avenue, Almaty 050060, Kazakhstan
Ilyas A. Akhmetollayev: National Center for Biotechnology,13/5 Kurgalzhyn Road, Astana 010000, Kazakhstan
Elina R. Maltseva: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86 Dosmukhamedov St., Almaty 050012, Kazakhstan; Tethys Scientific Society, 9 Microdisctrict 1/72, Almaty 050036, Kazakhstan
Yuriy A. Skiba: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86 Dosmukhamedov St., Almaty 050012, Kazakhstan; Tethys Scientific Society, 9 Microdisctrict 1/72, Almaty 050036, Kazakhstan
Seidigapbar M. Mamadaliyev: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan
Andrey M. Dmitrovskiy: Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan; M. Aikimbayev National Scientific Center for Especially Dangerous Infections, 14 Zhahanger St., Almaty 050054, Kazakhstan

Journal volume & issue: Vol. 14, no. 6
p. 102240

Abstract

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Ticks are involved in the circulation of a number of human pathogens, including spotted fever group (SFG) Rickettsia spp. and Coxiella burnetii. Little is known about the occurrence of these microorganisms in the southern region of Kazakhstan. In 2018–2022, a total of 726 ticks were collected from bitten humans, livestock, and vegetation in four oblasts of the southern region of Kazakhstan and subjected to DNA extraction. The overall infection rate of Coxiella spp. and Rickettsia spp. in the ticks was 3.3% (24/726) and 69.9% (300/429), respectively. Phylogenetic analysis of ompA and gltA genes revealed the presence of three pathogenic SFG rickettsiae: Candidatus R. tarasevichiae, R. aeschlimannii and R. raoultii in ticks collected from bitten humans. In addition, Candidatus R. barbariae was detected in six Rhipicephalus turanicus ticks for the first time in Kazakhstan. To determine the seroprevalence of C. burnetii infection, we performed a serological analysis of samples collected from 656 domestic ruminants (cattle, sheep, and goats) in the region. Overall, 23.5% (154/656) of the animals tested were positive for IgG against C. burnetii. Seroprevalence at the herd level was 54% (28/52). Goats (43%; 12/28; odds ratio (OD) = 28.9, p < 0.05) and sheep (31.9%; 137/430; OD = 18.1, p < 0.05) had higher seroprevalence than cattle (2.5%; 5/198). Among the risk factors considered in this study, age (p = 0.003) and the oblast in which the animals were sampled (p = 0.049) were statistically associated with seropostivity for Q fever in sheep, according to the results of multivariate logistic regression analysis. Seroprevalence ranged from 0% to 55.5% in animals in different districts of the southern region of Kazakhstan. Active C. burnetii bacteremia was detected in four of 154 (2.6%) seropositive animals. The data obtained provide strong evidence of the presence of pathogenic rickettsiae and C. burnetii in the southern region of Kazakhstan and emphasize the need to improve epidemiological surveillance in the region.

Published in Ticks and Tick-Borne Diseases

ISSN: 1877-9603 (Online)
Publisher: Elsevier
Country of publisher: Germany
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: https://www.sciencedirect.com/journal/ticks-and-tick-borne-diseases

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