Nature Communications (May 2022)

A genome-wide CRISPR-Cas9 knockout screen identifies essential and growth-restricting genes in human trophoblast stem cells

  • Chen Dong,
  • Shuhua Fu,
  • Rowan M. Karvas,
  • Brian Chew,
  • Laura A. Fischer,
  • Xiaoyun Xing,
  • Jessica K. Harrison,
  • Pooja Popli,
  • Ramakrishna Kommagani,
  • Ting Wang,
  • Bo Zhang,
  • Thorold W. Theunissen

DOI
https://doi.org/10.1038/s41467-022-30207-9
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 16

Abstract

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Here the authors perform a genome-wide CRISPR-Cas9 knockout screen to systematically identify and characterize essential and growth-restricting genes in human trophoblast cells. They identify TEAD1 as a key regulator that plays an important role in the specification, maintenance, and differentiation of the human trophoblast lineage by modulating chromatin architecture and gene expression.