<em>In vitro</em> multiplication of <em>Zantedeschia</em> spp. Treasure variety in Temporary Immersion Systems

Abstract

Callas (Zantedeschia spp.) are plants from Araceae family of a great interest as ornamental plants in pots and as cut flowers. Although they have been propagated by in vitro culture, there are few scientific studies on the micropropagation in temporary immersion systems. This work was carried out in order to multiply in vitro Zantedeschia spp. in Temporary Immersion Systems. Effect of different immersion frequencies and concentrations of Paclobutrazol (PBZ) on the proliferation and quality of shoots was determined. The integration of these experiments lead to obtain a higher coefficient of multiplication (9.7 shoots per explant) with a frequency of immersion every four hours and the supplement of 0.3 mg.l-1 of PBZ in the culture medium. Keywords: callas, growth retardants, immersion frecuency, ornamentals