Live-cell protein labelling with nanometre precision by cell squeezing

Alina Kollmannsperger; Armon Sharei; Anika Raulf; Mike Heilemann; Robert Langer; Klavs F. Jensen; Ralph Wieneke; Robert Tampé

doi:10.1038/ncomms10372

Nature Communications (Jan 2016)

Live-cell protein labelling with nanometre precision by cell squeezing

Alina Kollmannsperger,
Armon Sharei,
Anika Raulf,
Mike Heilemann,
Robert Langer,
Klavs F. Jensen,
Ralph Wieneke,
Robert Tampé

Affiliations

Alina Kollmannsperger: Institute of Biochemistry, Biocenter, Goethe-University Frankfurt
Armon Sharei: Department of Chemical Engineering, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT)
Anika Raulf: Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt
Mike Heilemann: Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt
Robert Langer: Department of Chemical Engineering, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT)
Klavs F. Jensen: Department of Chemical Engineering, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT)
Ralph Wieneke: Institute of Biochemistry, Biocenter, Goethe-University Frankfurt
Robert Tampé: Institute of Biochemistry, Biocenter, Goethe-University Frankfurt

DOI: https://doi.org/10.1038/ncomms10372
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

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The direct observation of intracellular processes through microscopy can provide key insight into biological pathways. Here the authors demonstrate the combination of microfluidic cell squeezing and fluorescent label delivery to native proteins for high throughput live cell super-resolution imaging.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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