Insights into the Metabolite Differentiation Mechanism Between Chinese Dry-Cured Fatty Ham and Lean Ham Through UPLC-MS/MS-Based Untargeted Metabolomics
Ruoyu Xie,
Xiaoli Wu,
Jun Hu,
Wenxuan Chen,
Ke Zhao,
Huanhuan Li,
Lihong Chen,
Hongying Du,
Yaqiong Liu,
Jin Zhang
Affiliations
Ruoyu Xie
College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China
Xiaoli Wu
State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Jun Hu
State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Wenxuan Chen
State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Ke Zhao
State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Huanhuan Li
State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Lihong Chen
State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Hongying Du
Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
Yaqiong Liu
College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China
Jin Zhang
State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
To understand the impact and mechanism of removing fat and skin tissue on the nutritional metabolism of Chinese dry cured ham, the differential metabolites (DMs) profile between lean ham (LH) and fatty ham (FH) was explored though untargeted metabolomics based on UPLC-MS/MS. The results showed significant differences of the metabolite profiles between FH and LH. A total of 450 defined metabolites were detected, and 266 metabolites among them had significantly different abundances between the two hams, mainly including organic acids and derivatives, and lipids and lipid-like molecules, as well as organoheterocyclic compounds. Furthermore, 131 metabolites were identified as DMs, among which 101 and 30 DMs showed remarkably higher contents in FH and LH, respectively. The further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that DMs can be mostly enriched in the pathways of ABC transporters, amino acid biosynthesis, protein digestion and absorption, aminoacyl-tRNA biosynthesis, and 2-oxocarboxylic acid metabolism. Moreover, the metabolic network of DMs revealed that the prominent DMs in FH, such as 9(S)-HODE, 9,10-EpOME, 13-Oxo-ODE, L-palmitoyl carnitine, and D-fructose, were primarily involved in the endogenous oxidation and degradation of fat and glycogen. Nevertheless, the dominant DMs in LH, such as 2-isopropylmalic acid, indolelactic acid, and hydroxyisocaproic acid, were mainly the microbial metabolites of amino acids and derivates. These findings could help us understand how fat-deficiency affects the nutritional metabolism of Chinese dry-cured hams from a metabolic perspective.
