DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system

Ashton C. Trotman-Grant; Mahmood Mohtashami; Joshua De Sousa Casal; Elisa C. Martinez; Dylan Lee; Sintia Teichman; Patrick M. Brauer; Jianxun Han; Michele K. Anderson; Juan Carlos Zúñiga-Pflücker

doi:10.1038/s41467-021-25245-8

Nature Communications (Aug 2021)

DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system

Ashton C. Trotman-Grant,
Mahmood Mohtashami,
Joshua De Sousa Casal,
Elisa C. Martinez,
Dylan Lee,
Sintia Teichman,
Patrick M. Brauer,
Jianxun Han,
Michele K. Anderson,
Juan Carlos Zúñiga-Pflücker

Affiliations

Ashton C. Trotman-Grant: Department of Immunology, University of Toronto
Mahmood Mohtashami: Sunnybrook Research Institute
Joshua De Sousa Casal: Department of Immunology, University of Toronto
Elisa C. Martinez: Sunnybrook Research Institute
Dylan Lee: Sunnybrook Research Institute
Sintia Teichman: Department of Immunology, University of Toronto
Patrick M. Brauer: Sunnybrook Research Institute
Jianxun Han: Sunnybrook Research Institute
Michele K. Anderson: Department of Immunology, University of Toronto
Juan Carlos Zúñiga-Pflücker: Department of Immunology, University of Toronto

DOI: https://doi.org/10.1038/s41467-021-25245-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

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T cells derived from stem cells can be harnessed for regenerative medicine and cancer immunotherapy, but current technologies limit production and translation. Here, the authors present a serum-free, stromal-cell free DLL4-coated microbead method for the scalable production of T-lineage cells from multiple sources of stem cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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