Transcriptome analysis reveals a potential regulatory mechanism of the lnc-5423.6/IGFBP5 axis in the early stages of mouse thymic involution

Li Bingxin; Ye Yaqiong; Hong Longsheng; Li Wanyan; Wu Qingru; Liu Wenjun; Ma Yongjiang; Xu Danning; Li Yugu

doi:10.3724/abbs.2023042

Acta Biochimica et Biophysica Sinica (Apr 2023)

Transcriptome analysis reveals a potential regulatory mechanism of the lnc-5423.6/IGFBP5 axis in the early stages of mouse thymic involution

Li Bingxin,
Ye Yaqiong,
Hong Longsheng,
Li Wanyan,
Wu Qingru,
Liu Wenjun,
Ma Yongjiang,
Xu Danning,
Li Yugu

Affiliations

Li Bingxin: ["College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China","College of Animal Science & Technology, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"]
Ye Yaqiong: ["School of Life Science and Engineering, Foshan University, Foshan 528000, China"]
Hong Longsheng: ["College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"]
Li Wanyan: ["College of Animal Science & Technology, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"]
Wu Qingru: ["College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"]
Liu Wenjun: ["College of Animal Science & Technology, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"]
Ma Yongjiang: ["College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"]
Xu Danning: ["College of Animal Science & Technology, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"]
Li Yugu: ["College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"]

DOI: https://doi.org/10.3724/abbs.2023042
Journal volume & issue: Vol. 55
pp. 548 – 560

Abstract

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Age-related thymic involution is one of the significant reasons for induced immunity decline. Recent evidence has indicated that lncRNAs are widely involved in regulating organ development. However, the lncRNA expression profiles in mouse thymic involution have not been reported. In this study, we collect mouse thymus at the ages of 1 month, 3 months, and 6 months for sequencing to observe the lncRNA and gene expression profiles in the early stages of thymic involution. Through bioinformatics analysis, a triple regulatory network of lncRNA-miRNA-mRNA that contains 29 lncRNAs, 145 miRNAs and 12 mRNAs that may be related to thymic involution is identified. Among them, IGFBP5 can reduce the viability, inhibit proliferation and promote apoptosis of mouse medullary thymic epithelial cell line 1 (MTEC1) cells through the p53 signaling pathway. In addition, miR-193b-3p can alleviate MTEC1 cell apoptosis by targeting IGFBP5. Notably, lnc-5423.6 can act as a molecular sponge of miR-193b-3p to regulate the expression of IGFBP5. In summary, lnc-5423.6 enhances the expression of IGFBP5 by adsorption of miR-193b-3p, thereby promoting MTEC1 cell apoptosis.

Published in Acta Biochimica et Biophysica Sinica

ISSN: 1672-9145 (Print); 1745-7270 (Online)
Publisher: China Science Publishing & Media Ltd.
Country of publisher: China
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry; Science: Biology (General): Genetics
Website: https://www.sciengine.com/ABBS/home

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