CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

• Xiao-Wen Fan,
• Zi-Fan Gao,
• Dong-Dong Ling,
• De-Hui Wang,
• Ying Cui,
• Hui-Qun Du,
• Chun-Lin Li,
• Xing Zhou

Affiliations

Xiao-Wen Fan

Jinling Clinical Medical College, Nanjing University of Chinese Medicine

Zi-Fan Gao

Jinling Clinical Medical College, Nanjing University of Chinese Medicine

Dong-Dong Ling

Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University

De-Hui Wang

Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University

Ying Cui

Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University

Hui-Qun Du

Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University

Chun-Lin Li

Department of Health Medicine, the Eighth Medical Center of PLA General Hospital

Xing Zhou

Jinling Clinical Medical College, Nanjing University of Chinese Medicine