Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications

Marius Rădulescu; Alina Maria Holban; Laurențiu Mogoantă; Tudor-Adrian Bălşeanu; George Dan Mogoșanu; Diana Savu; Roxana Cristina Popescu; Oana Fufă; Alexandru Mihai Grumezescu; Eugenia Bezirtzoglou; Veronica Lazar; Mariana Carmen Chifiriuc

doi:10.3390/molecules21060761

Molecules (Jun 2016)

Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications

Marius Rădulescu,
Alina Maria Holban,
Laurențiu Mogoantă,
Tudor-Adrian Bălşeanu,
George Dan Mogoșanu,
Diana Savu,
Roxana Cristina Popescu,
Oana Fufă,
Alexandru Mihai Grumezescu,
Eugenia Bezirtzoglou,
Veronica Lazar,
Mariana Carmen Chifiriuc

Affiliations

Marius Rădulescu: Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Alina Maria Holban: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Laurențiu Mogoantă: Research Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, PetruRares Street, No. 2, 200349 Craiova, Romania
Tudor-Adrian Bălşeanu: Research Center for Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova 2 PetruRareş Street, 200349 Craiova, Romania
George Dan Mogoșanu: Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, PetruRares Street, No. 2, 200349 Craiova, Romania
Diana Savu: Department of Life and Environmental Physics, “HoriaHulubei” National Institute of Physics and Nuclear Engineering, Magurele, 077125 Bucharest, Romania
Roxana Cristina Popescu: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Oana Fufă: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Alexandru Mihai Grumezescu: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Eugenia Bezirtzoglou: Laboratory of Microbiology, Biotechnology and Hygiene, Department of Food Science and Technology, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, Greece
Veronica Lazar: Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania
Mariana Carmen Chifiriuc: Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania

DOI: https://doi.org/10.3390/molecules21060761
Journal volume & issue: Vol. 21, no. 6
p. 761

Abstract

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The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic. Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques. In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line. The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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