Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing

Louise H.W. Kung; Lisa Sampurno; Christopher B. Little; Shireen R. Lamandé; John F. Bateman

Stem Cell Research (Jan 2021)

Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing

Louise H.W. Kung,
Lisa Sampurno,
Christopher B. Little,
Shireen R. Lamandé,
John F. Bateman

Affiliations

Louise H.W. Kung: Murdoch Children’s Research Institute, Parkville, Victoria, Australia
Lisa Sampurno: Murdoch Children’s Research Institute, Parkville, Victoria, Australia
Christopher B. Little: Raymond Purves Bone and Joint Research Laboratories, Kolling Institute of Medical Research, Institute of Bone and Joint Research, University of Sydney, St Leonards, New South Wales, Australia
Shireen R. Lamandé: Murdoch Children’s Research Institute, Parkville, Victoria, Australia; Department of Paediatrics, University of Melbourne, Australia
John F. Bateman: Murdoch Children’s Research Institute, Parkville, Victoria, Australia; Department of Paediatrics, University of Melbourne, Australia; Corresponding author at: Murdoch Children’s Research Institute, Parkville, Victoria, Australia.

Journal volume & issue: Vol. 50
p. 102118

Abstract

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miR-26b has been implicated in a wide range of human diseases, including cancer, diabetes, heart disease, Alzheimer’s disease and osteoarthritis. To provide a tool to explore the importance of miR-26b in this broad context, we have generated and characterized a homozygous miR-26b stem-loop knockout human iPSC line. This gene-edited line exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. This iPSC line will be valuable for studies investigating disease mechanisms and testing therapeutic strategies in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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