PHBV/PCL Microparticles for Controlled Release of Resveratrol: Physicochemical Characterization, Antioxidant Potential, and Effect on Hemolysis of Human Erythrocytes

Jessica Bitencourt Emilio Mendes; Manoela Klüppel Riekes; Viviane Matoso de Oliveira; Milton Domingos Michel; Hellen Karine Stulzer; Najeh Maissar Khalil; Sônia Faria Zawadzki; Rubiana Mara Mainardes; Paulo Vitor Farago

doi:10.1100/2012/542937

The Scientific World Journal (Jan 2012)

PHBV/PCL Microparticles for Controlled Release of Resveratrol: Physicochemical Characterization, Antioxidant Potential, and Effect on Hemolysis of Human Erythrocytes

Jessica Bitencourt Emilio Mendes,
Manoela Klüppel Riekes,
Viviane Matoso de Oliveira,
Milton Domingos Michel,
Hellen Karine Stulzer,
Najeh Maissar Khalil,
Sônia Faria Zawadzki,
Rubiana Mara Mainardes,
Paulo Vitor Farago

Affiliations

Jessica Bitencourt Emilio Mendes: Postgraduate Program in Pharmaceutical Sciences, Department of Pharmaceutical Sciences, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenue, 84030-900 Ponta Grossa, Brazil
Manoela Klüppel Riekes: Laboratory of Quality Control, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, P.O. Box 476, 88040-900 Florianópolis, Brazil
Viviane Matoso de Oliveira: Laboratory of Nanotechnology, Department of Pharmacy, State University of the Center-West, 3 Simeão Camargo Varela de Sá St, 85040-080 Guarapuava, Brazil
Milton Domingos Michel: Department of Materials Engineering, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenue, 84030-900 Ponta Grossa, Brazil
Hellen Karine Stulzer: Laboratory of Quality Control, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, P.O. Box 476, 88040-900 Florianópolis, Brazil
Najeh Maissar Khalil: Laboratory of Nanotechnology, Department of Pharmacy, State University of the Center-West, 3 Simeão Camargo Varela de Sá St, 85040-080 Guarapuava, Brazil
Sônia Faria Zawadzki: Department of Chemistry, Federal University of Paraná, P.O. Box 19081, 81531-990 Curitiba, Brazil
Rubiana Mara Mainardes: Laboratory of Nanotechnology, Department of Pharmacy, State University of the Center-West, 3 Simeão Camargo Varela de Sá St, 85040-080 Guarapuava, Brazil
Paulo Vitor Farago: Postgraduate Program in Pharmaceutical Sciences, Department of Pharmaceutical Sciences, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenue, 84030-900 Ponta Grossa, Brazil

DOI: https://doi.org/10.1100/2012/542937
Journal volume & issue: Vol. 2012

Abstract

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Microparticles of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) containing resveratrol were successfully prepared by simple emulsion/solvent evaporation. All formulations showed suitable encapsulation efficiency values higher than 80%. PHBV microparticles revealed spherical shape with rough surface and presence of pores. PCL microparticles were spherically shaped with smooth surface. Fourier-transformed infrared spectra demonstrated no chemical bond between resveratrol and polymers. X-ray powder diffraction patterns and differential scanning calorimetry analyses indicated that microencapsulation led to drug amorphization. These PHBV/PCL microparticles delayed the dissolution profile of resveratrol. Release profiles were better fitted to biexponential equation. The hypochlorous-acid-scavenging activity and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation discoloration assay confirmed that the antioxidant activity of PHBV/PCL microparticles was kept, but was dependent on the microparticle morphology and dissolution profile. Resveratrol-loaded PHBV/PCL microparticles showed no cytotoxic effect on red blood cells.

Published in The Scientific World Journal

ISSN: 2356-6140 (Print); 1537-744X (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Technology; Medicine; Science
Website: https://onlinelibrary.wiley.com/journal/8086

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