Peculiarities of plant adaptation of interspecific hybrid Betula ex vitro

Abstract

Microclonal propagation as one of the methods of biotechnology allows obtaining genetically homogeneous plants during the year from a minimum amount of donor material. Adaptation of plants ex vitro to environmental conditions is the final and important stage of microclonal propagation. The purpose of the study is to determine the optimal mode of plant adaptation of triploid interspecific hybrid Betula ex vitro to environmental conditions. Biotechnological (microclonal propagation) and statistical (arithmetic mean, standard error, one-way analysis of variance (ANOVA)) methods were used for research. The study was carried out in the research of Plant Biotechnology Laboratory of the Separated Subdivision of National University of Life and Environmental Sciences of Ukraine “Boyarka Forest Research Station” during 2019-2023. According to research, the MS nutrient medium with the addition of 0.25 mg∙l -1 of kinetin stimulated the active proliferation of microshoots and root system. Optimum plant development took place on the nutrient medium vitro for a 25-30- day cycle of cultivation, which contributed to successful adaptation to environmental conditions. The proportion of adapted Betula ex vitro hybrid plants on agroperlite substrate was more than 60% for 30 days. Significant plant viability (more than 80%) was obtained under the conditions of preliminary exposure of the plant root system in auxin solution (1.0 mg/lIAA, 1.0 mg/l NAA, 1.0 mg/lIBA) for 25-30 minutes followed by daily spraying of leaves with 20% glycerin for 15-20 days. Adapted plants had typical pigmentation without signs of chlorosis and vitrification. In the spring, the survival rate of plants under environmental conditions was more than 80% and indicated a high level of adaptability after in vitro. The developed protocol for the adaptation of the Betula ex vitro hybrid allows obtaining high plant survival in environmental conditions. The practical value of the research is obtaining plants adapted to environmental conditions ex vitro, which in the future can be used to qualitatively enrich the range of artificial plantings in settlements

Keywords

• culture in vitro
• birch
• microclonal propagation
• substrate
• viability