SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly

Susan K. Vester; Rolle Rahikainen; Irsyad N. A. Khairil Anuar; Rory A. Hills; Tiong Kit Tan; Mark Howarth

doi:10.1038/s41467-022-31193-8

Nature Communications (Jun 2022)

SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly

Susan K. Vester,
Rolle Rahikainen,
Irsyad N. A. Khairil Anuar,
Rory A. Hills,
Tiong Kit Tan,
Mark Howarth

Affiliations

Susan K. Vester: Department of Biochemistry, University of Oxford
Rolle Rahikainen: Department of Biochemistry, University of Oxford
Irsyad N. A. Khairil Anuar: Department of Biochemistry, University of Oxford
Rory A. Hills: Department of Biochemistry, University of Oxford
Tiong Kit Tan: MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Mark Howarth: Department of Biochemistry, University of Oxford

DOI: https://doi.org/10.1038/s41467-022-31193-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

Read online

The SpyCatcher-SpyTag system allows protein anchoring and nanoassembly. Here, the authors engineer SpySwitch, a dually switchable Catcher which allows gentle purification of SpyTagged proteins prior to downstream applications such as the assembly of virus-like particles.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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