Nature Communications (Jan 2019)

Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions

  • Hyunjin Jung,
  • Seong-Wook Kim,
  • Minsoo Kim,
  • Jongryul Hong,
  • Daseuli Yu,
  • Ji Hye Kim,
  • Yunju Lee,
  • Sungsoo Kim,
  • Doyeon Woo,
  • Hee-Sup Shin,
  • Byung Ouk Park,
  • Won Do Heo

DOI
https://doi.org/10.1038/s41467-018-08282-8
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

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Most approaches to control gene expression in vivo require generation of knock-in mouse lines and often lack spatiotemporal control. Here the authors develop a photo-activatable Flp recombinase system and demonstrate its use by controlling object-exploration behavior in mice through Cav3.1 silencing.