Actinoquinazolinone, a New Quinazolinone Derivative from a Marine Bacterium <i>Streptomyces</i> sp. CNQ-617, Suppresses the Motility of Gastric Cancer Cells
Sultan Pulat,
Da-Ae Kim,
Prima F. Hillman,
Dong-Chan Oh,
Hangun Kim,
Sang-Jip Nam,
William Fenical
Affiliations
Sultan Pulat
College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea
Da-Ae Kim
Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea
Prima F. Hillman
Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea
Dong-Chan Oh
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea
Hangun Kim
College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea
Sang-Jip Nam
Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea
William Fenical
Center of Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093-0204, USA
A HPLC-UV guided fractionation of the culture broth of Streptomyces sp. CNQ-617 has led to the isolation of a new quinazolinone derivative, actinoquinazolinone (1), as well as two known compounds, 7-hydroxy-6-methoxy-3,4-dihydroquinazolin-4-one (2) and 7-methoxy-8-hydroxy cycloanthranilylproline (3). The interpretation of 1D, 2D NMR, and MS spectroscopic data revealed the planar structure of 1. Furthermore, compound 1 suppressed invasion ability by inhibiting epithelial–mesenchymal transition markers (EMT) in AGS cells at a concentration of 5 µM. In addition, compound 1 decreased the expression of seventeen genes related to human cell motility and slightly suppressed the signal transducer and activator of the transcription 3 (STAT3) signal pathway in AGS cells. Together, these results demonstrate that 1 is a potent inhibitor of gastric cancer cells.
