Nature Communications (Dec 2022)

Mechanism of human Lig1 regulation by PCNA in Okazaki fragment sealing

  • Kerry Blair,
  • Muhammad Tehseen,
  • Vlad-Stefan Raducanu,
  • Taha Shahid,
  • Claudia Lancey,
  • Fahad Rashid,
  • Ramon Creuhet,
  • Samir M. Hamdan,
  • Alfredo De Biasio

DOI
https://doi.org/10.1038/s41467-022-35475-z
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 15

Abstract

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In this work, Blair and co-authors used cryo-EM and in vitro assays to show that human Ligase 1 (Lig1) and Flap Endonuclease 1 (FEN1) form a toolbelt with the sliding clamp PCNA coordinating the sealing of Okazaki fragments.