Effect of Physical and Enzymatic Pre-Treatment on the Nutritional and Functional Properties of Fermented Beverages Enriched with Cricket Proteins
Chaima Dridi,
Mathieu Millette,
Blanca Aguilar,
Johanne Manus,
Stephane Salmieri,
Monique Lacroix
Affiliations
Chaima Dridi
INRS Armand-Frappier Health Biotechnology Research Centre, Research Laboratories in Sciences, Applied to Food (RESALA), Canadian Irradiation Centre (CIC), Institute of Nutrition and Functional Foods (INAF), 531 Boulevard des Prairies, Laval, QC H7V 1B7, Canada
Mathieu Millette
Bio-K Plus International Inc., a Kerry Company, Preclinical Research Division, 495 Armand-Frappier Blvd, Laval, QC H7V 4B3, Canada
Blanca Aguilar
Research Laboratory of Industrial Microbiology, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, 1421, Blvd, Marcelino Garcia Barragan, Col. Olímpica, Guadalajara 44430, Jalisco, Mexico
Johanne Manus
INRS Armand-Frappier Health Biotechnology Research Centre, Research Laboratories in Sciences, Applied to Food (RESALA), Canadian Irradiation Centre (CIC), Institute of Nutrition and Functional Foods (INAF), 531 Boulevard des Prairies, Laval, QC H7V 1B7, Canada
Stephane Salmieri
INRS Armand-Frappier Health Biotechnology Research Centre, Research Laboratories in Sciences, Applied to Food (RESALA), Canadian Irradiation Centre (CIC), Institute of Nutrition and Functional Foods (INAF), 531 Boulevard des Prairies, Laval, QC H7V 1B7, Canada
Monique Lacroix
INRS Armand-Frappier Health Biotechnology Research Centre, Research Laboratories in Sciences, Applied to Food (RESALA), Canadian Irradiation Centre (CIC), Institute of Nutrition and Functional Foods (INAF), 531 Boulevard des Prairies, Laval, QC H7V 1B7, Canada
The aim of this study was to evaluate the effects of γ-irradiation (IR), ultrasound (US), and combined treatments of ultrasound followed by γ-irradiation (US-IR), ultrasound followed by enzymatic hydrolysis with and without centrifugation (US-E and US-EWC, respectively), and ultrasound followed by γ-irradiation and enzymatic hydrolysis (US-IRE), on the digestibility and the nutritional value of fermented beverages containing probiotics. Results showed that US (20 min), IR (3 kGy) and US-IR (tUS = 20 min, dose = 3 kGy) treatments raised protein solubility from 11.5 to 21.5, 24.3 and 29.9%, respectively. According to our results, these treatments were accompanied by the increased amount of total sulfhydryl groups, surface hydrophobicity and changes to the secondary structure of the proteins measured by Fourier-transform infrared spectroscopy (FTIR). Fermented probiotic beverages, non-enriched (C) and enriched with untreated (Cr) or treated cricket protein with combined treatments were also evaluated for their in vitro protein digestibility. Results showed that the soluble fraction of US-IRE fermented beverage had the highest digestibility (94%) as compared to the whole fermented tested beverages. The peptides profile demonstrated that US-IRE had a low proportion of high molecular weight (MW) peptides (0.7%) and the highest proportion of low MW peptides by over 80% as compared to the other treatments.
